FIGURE

Fig. 4

ID

ZDB-FIG-080501-23

Publication

diIorio et al., 2002 - Sonic hedgehog is required early in pancreatic islet development

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Fig. 4

Insulin and Pdx-1 expression after shh overexpression in WT embryos. Left: mRNA control-injected embryos. Right; shh mRNA-injected embryos. After mRNA injection at the two- to four-cell stage, embryos developed for 24 or 48 hpf and were subjected to whole-mount in situ analysis using the insulin or Pdx-1 probe. (A, C, E) Control GFP mRNA-injected embryos hybridized at 24 hpf with the insulin probe. Positive cells are centered ventral to somite 4 (4). (B, D, F) Shh mRNA-injected embryos hybridized with the insulin probe. Note the anterior extension of labeled cells. (G) Control β-gal mRNA-injected embryo hybridized with the Pdx-1 probe. (H) Shh mRNA-injected embryo hybridized with the Pdx-1 probe. (A, B, E, F) Ventral views. (C, D) Lateral views. (G, H) Dorsal views.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 244(1), diIorio, P.J., Moss, J.B., Sbrogna, J.L., Karlstrom, R.O., and Moss, L.G., Sonic hedgehog is required early in pancreatic islet development, 75-84, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.