Fig. 5

Craniofacial and Heart Defects upon NS or LS RNA Injection
(A,C) Extension of anterior structures (black asterisk) was impaired and the eyes were spaced wider apart (black bar) in NS-Shp2–injected embryos (D61G, 150 pg) (C) than in the noninjected control (A). The figure is representative of defects associated with all NS- and LS-Shp2–injected embryos. (B,D) Alcian blue staining of the cartilage in the head of NS-Shp2 injected embryos (D) compared to noninjected control (B). Black asterisk, Meckel's cartilage; red asterisk, ceratohyal. (E–K) NS- and LS-induced heart defects. (E–H) Morphology of representative 3 dpf embryos with (E) noninjected control, (F) mild, (G) intermediate, and (H) severe heart defect. Phenotypes evoked by NS- and LS-Shp2 were indistinguishable. (I–K) cmlc2 in situ hybridization marks the heart. (I) Noninjected control with normal heart jogging (red asterisk). (J) Injected embryo showing defective cardiac jogging (red asterisk). (K) Quantification of heart jogging of NS- or LS-Shp2 RNA–injected embryos stained with cmlc2, depicted as percent non-jogging.