Fig. 3

psoriasis acts non-cell-autonomously. A: Control transplant showing wild-type cells transplanted into a wild-type host epidermis. B: psoriasis mutant cells transplanted into a wild-type host contribute to epidermis and display wild-type morphology. C: Wild-type cells transplanted into a psoriasis mutant contribute to epidermal aggregates. D: Overview of transplantation experiments combined with 5′-bromodeoxyuridine (BrdU) assays. Cells from fluorescein-labeled donors were transplanted to unlabeled hosts at 4 hours postfertilization (hpf). At 3.5 days postfertilization (dpf), cell proliferation was assayed by BrdU-labeling donors and hosts. E-G: Brightfield images of host embryos. H-J: Merged images showing transplanted cells (green) and BrdU-positive cells (red) are shown below the corresponding brightfield images. E,H: In wild-type to wild-type transplants, transplanted epidermal cells are BrdU-negative, showing that the wild-type embryo and transplanted cells have halted proliferation. F,I: psoriasis cells transplanted into a wild-type host are BrdU-negative. G,J: A psoriasis host containing a BrdU-positive wild-type epidermal cell (arrow) at 80 hpf. Anterior is to the left; all embryos shown at 80 hpf. Scale bars = 100 μm.