arginine vasotocin-neurophysin (avt) mRNA expression in the zebrafish embryo.A-F: Expression of avt mRNA (dark blue) determined by whole-mount in situ hybridization (ISH). G-I: Expression of avt (purple) and isotocin (ist) mRNA (red) determined by double-label ISH. A-C and J-L are lateral views, dorsal is up, and anterior to the left. D,E: Frontal views, dorsal is up. F-I: Ventral views, anterior is up. The eyes have been removed from the embryo show in C. The ages of the embryos are indicated in the lower left corner in hours postfertilization (hpf) or days postfertilization (dpf). The probes are indicated in the upper right corner. The avt-expressing cell groups in the ventral hypothalamus are indicated by black arrows, whereas the avt cell group in the dorsal preoptic area is indicated with a black arrowhead. The ist-expressing cells are indicated with a white arrowhead. I is a higher magnification of the region outlined by the white box in H. hy, hypothalamus; m, midbrain; t, telencephalon. Scale bars = 100 μm.
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