FIGURE

Fig. 6

ID

ZDB-FIG-080402-6

Publication

Blechman et al., 2007 - Specification of hypothalamic neurons by dual regulation of the homeodomain protein Orthopedia

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Fig. 6

Regulation of the Otp protein by PACAP/PAC1 signaling pathway. (A-H) Embryos were injected with either a control pac1 missense morpholino (Control, A,B), or with antisense morpholinos directed against pac1 (pac1MO, C,D), otpa (otpaMO, E,F) or combination of pac1+otpa (pac1MO+otpaMO, G,H) and were thereafter (52 hpf) subjected to either in situ hybridization with an otpb-directed probe (A,C,E,G), or to immunohistochemistry with an anti-Otp antibody (B,D,F,H). Inset in panel D shows western blot analysis of nuclear-enriched protein extracts from control and pac1MO-injected embryos using antibodies directed to either Otp or the nuclear protein Rcc1 (internal protein loading control). The activity of each morpholino was evaluated by anti-tyrosine hydroxylase (TH) immunostaining. Scale bars: 100 μm in A,C,E,F; 50 μm in B,D,F,H.

Expression Data

Gene:	otpb
Fish:	WT WT + MO1-adcyap1r1b WT + MO1-otpa WT + MO1-adcyap1r1b + MO1-otpa
Knockdown Reagents:	MO1-adcyap1r1b MO1-otpa
Anatomical Terms:	caudal tuberculum diencephalon hindbrain hypothalamus preoptic area
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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