FIGURE

Fig. 6

ID

ZDB-FIG-080326-95

Publication

Sato-Maeda et al., 2008 - Position fine-tuning of caudal primary motoneurons in the zebrafish spinal cord

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Fig. 6

Aberrant exit points of secondary motoneuron (SMN) axons in Nrp1a-knockdown embryos. (A) Axons of primary motoneurons (PMNs) and SMNs at 48 hpf in a control MO-injected embryo. PMN axons were labeled with a brown stain, recognized by mAb Znp1, but not by Zn5 (Zeller et al., 2002). SMN axons were stained in blue-black, recognized by Zn5 (Fashena and Westerfield, 1999), regardless of Znp1 reactivity. SMN axons extended ventrally along with PMN axons using an exit point common to PMN and SMN. (B) Double exit phenotype of SMN axons was observed in Nrp1a-knockdown embryos at 48 hpf. Generally, one axonal bundle of SMNs was thicker (arrowhead) than the others (arrow), regardless of its anterior or posterior position. (C) Another example of a double exit phenotype of SMN. Scale bar: 50 μm.

Expression Data

Gene:	alcama
Fish:	WT WT + MO2-nrp1a
Knockdown Reagent:	MO2-nrp1a
Anatomical Term:	secondary motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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