FIGURE

Fig. S2

ID

ZDB-FIG-080325-80

Publication

Bessa et al., 2008 - meis1 regulates cyclin D1 and c-myc expression, and controls the proliferation of the multipotent cells in the early developing zebrafish eye

Other Figures

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Fig. S2

meis1-MO injection reduces rhombomeres-3 krox20 expression specifically. (A) Ctrl-MO has no effect on krox20 expression in rhombomeres 3 (r3) or 5 (r5). (B) meis1-MO embryos show a specific reduction of r3 krox20 expression (red arrowhead). (C) Injection of a meis1, 2.2, 3 and 4 MO cocktail results in an even stronger inhibition of r3 krox20 expression (red arrowheads). (D) This effect is partially rescued by injection of GFP-meis1 mRNA (meis1GFP) (black arrows). GFP-meis1 is insensitive to meis1-MO, since the N-terminal tagging of meis1 with GFP destroys the meis1-MO target sequence in the GFP-meis1 mRNA. These results phenocopy the reported effect on r3 krox20 expression caused by injection of a dominant-negative Meis form (Waskiewicz et al., 2001) and thus confirm the biological specificity of the Meis mopholinos used in this study.

Expression Data

Gene:	egr2b
Fish:	WT WT + MO1-meis1b
Knockdown Reagent:	MO1-meis1b
Anatomical Terms:	rhombomere 3 rhombomere 5
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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