FIGURE

Fig. 7

ID

ZDB-FIG-080325-26

Publication

Hendricks et al., 2008 - Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation

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Fig. 7

wnt4a and dnRyk affect commissure formation. Lateral (A) and dorsal (B) view of a 48 hpf embryo, showing expression of wnt4a in the medial habenula. (C) A 3 dpf embryo after injection with a morpholino (MO1) to wnt4a. Habenular afferents have entered the habenula and reached the medial edge (arrows) but have not crossed the roof plate. Axons have innervated the habenula neuropil (arrowhead), which normally occurs only after the commissure has formed. (D) An embryo at 3 dpf after unilateral electroporation with HuC:Gal4/UAS:dnRyk-EGFP at 1 dpf. Abnormal loops and branches are seen within the commissure (n = 15). (E, F) Time series of individual axons show that growth cones reach the contralateral boundary, then bifurcate (arrowheads), turn around and recross the midline (see Supplementary Movies 5 and 6). Time series in (E) was taken without a heated stage, causing slower growth. Time is h:min.

Expression Data

Gene:	wnt4
Fish:	WT
Anatomical Term:	habenula
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt4 WT + MO3-wnt4
Knockdown Reagents:	MO1-wnt4 MO3-wnt4
Observed In:	habenular commissure
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 37(2), Hendricks, M., Mathuru, A.S., Wang, H., Silander, O., Kee, M.Z., and Jesuthasan, S., Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation, 271-283, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.