FIGURE

Fig. 5

ID

ZDB-FIG-080325-118

Publication

Serluca, 2008 - Development of the proepicardial organ in the zebrafish

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Fig. 5

Bifid hearts are each associated with their own proepicardial organ. Face views of buffer-injected (A) and milMO-injected (B) embryos stained with MF20 against myosin heavy chain (brown) and wt1 to mark the PEO (blue). A PEO cluster is visible at near the AV junction in buffer-injected embryos (arrow in panel A) while each heart in the milMO embryo is associated with a cluster of wt1-positive PEO (arrows in panel B). (C) Dorsal and (D) dorsolateral views on embryos injected with casMO. The nephric wt1 expression is indicated by the arrowheads and lies ventral to the anterior somites stained with MF20 (brown). Large, diffuse clusters of wt1 cells can be seen in the lateral positions (arrows). Dorsal views are shown because these cells fail to reach their more ventral positions next to the heart, seen here below the plane of focus in brown (asterisk). Anterior is to the left in panels C and D.

Expression Data

Gene:	wt1a
Fish:	WT WT + MO2-s1pr2 WT + MO2-sox32
Knockdown Reagents:	MO2-s1pr2 MO2-sox32
Anatomical Term:	heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-s1pr2 WT + MO2-sox32
Knockdown Reagents:	MO2-s1pr2 MO2-sox32
Observed In:	heart
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Serluca, F.C., Development of the proepicardial organ in the zebrafish, 18-27, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.