Fig. 4

Antisense mediated knockdown of oc3 and hnf6 has a similar effect on bile duct function and development as knockdown of oc3 alone. A-D: PED6 uptake in 5-dpf larvae injected at the one-cell stage with vehicle (A), oc3 morpholino antisense oligonucleotide (B), hnf6 morpholino (C), or oc3 and hnf6 morpholinos together (D). Note that biliary secretion, measured as gallbladder fluorescence, is comparably reduced in Oc3-deficient (B), Hnf6-deficient (C), and Oc3/Hnf6-deficient larvae (D) relative to control (A). E-I: Confocal projections of cytokeratin immunostained 5-dpf whole-mount livers injected with vehicle control (E), oc3 morpholino antisense oligonucleotide (F), hnf6 morpholino (G), oc3 and hnf6 morpholinos together (H), or oc3 morpholino and hnf6 mRNA (I). The biliary phenotype of Oc3-deficient larva (F) is much more pronounced than that of the Hnf6-deficient larva (G). Rescue of the Oc3-deficient biliary phenotype by hnf6 mRNA injection is evident when I is compared to F. Note the lack of ectopic cytokeratin and the presence of intact ductular structures in I vs. F.