FIGURE

Fig. 3

ID

ZDB-FIG-071227-15

Publication

Esguerra et al., 2007 - Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination

Other Figures

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Fig. 3

Randomized LR gene expression and organ laterality in Ttrap^MO embryos. (A-E) Left-sided, right-sided, bilateral or absent/reduced marker expression. For all panels, dorsal views, anterior at top; single arrows denote sidedness, double arrows bilateral expression. (A) bmp4 in cardiac primordium (22 ss). Dashed white line denotes midline (L, left; R, right). (B,C) pitx2 and spaw in LPM (22 ss). (D) foxA3, 48 hpf (orange arrow, liver, liv; red arrow, pancreas, pa). (E) lft1 in the diencephalon (22-24 ss). Dashed line indicates midline; arrows indicate the relevant altered expression domain. (F) Bar graphs showing percentage of embryos with sided expression of these markers within each phenotypic category (y-axis), in control^MO and Ttrap^MO embryos (x-axis). Blue bars, left-sided expression; red, right-sided; yellow, bilateral; purple, absent/reduced expression. n=total embryos from two experiments.

Expression Data

Genes:	bmp4 foxa3 lft1 pitx2 spaw
Fish:	WT WT + MO1-tdp2b
Knockdown Reagent:	MO1-tdp2b
Anatomical Terms:	diencephalon gut heart primordium lateral plate mesoderm liver pancreas
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tdp2b
Knockdown Reagent:	MO1-tdp2b
Observed In:	determination of left/right symmetry
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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