GFP Transgenic Lines Provide Good Landmarks in the Neural Plate (A–D) pax6a (red) and her5pac:egfp (green) are expressed in adjacent neural plate territories at bud stage (A and C) and 1-somite stage (B and D); The same embryos are shown in dorsal (A and B) and lateral views (C and D), rostral to the left. (C and D) show dynamic changes in the neural plate positioning the rostral midbrain dorsal to the caudal diencephalon; (E and F) Dorsal views, rostral to the left of neural plates showing barhl2 (red) and her5pac:egfp (green) expression revealing a shape change of the rostral diencephalon from a mild V-shape (E) at early bud stage to a straight line (F) at late bud stage; (G and H) Dorsal views, rostral to the left of neural plates showing irx7 (red) + her5pac:egfp (green) expression, visualizing the domain of the caudal diencephalon anterior to the midbrain at early bud stage (G) and late bud stage (H).
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