FIGURE

Fig. 4

ID

ZDB-FIG-071204-28

Publication

Li et al., 2007 - Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Neural crest and placode derivatives are absent or reduced in tfap2a/c deficient embryos. (A, B) Lateral views of 28 hpf embryos processed for anti-HNK1 (Zn12 antibody) immunoreactivity (IR), revealing the presence of cranial ganglia neurons in panel A, a control MO-injected wild-type embryo, and their absence in panel B, a tfap2a^-/c^MO embryo (9 of 10 tfap2a^-/c^MO embryos). (C–F) Lateral and (G–J) ventral views of 72 hpf embryos processed for anti-Hu IR. (C, E, G, I) In control embryos anti-Hu IR reveals panel C, cranial ganglia surrounding the otic vesicle (ot), (E), dorsal root ganglia (drg) in the trunk, (G), sympathetic neurons at vagal level, and (I), proximal enteric neurons, adjacent to gut (g), also at vagal level. (D, F, H, J) All of these neurons appear to be absent from a tfap2a^-/c^MO embryo (8 of 8 tfap2a^-/c^MO embryos). (K, L) Dorsal and (M, N) lateral views of 28 hpf embryos processed to reveal foxd3 expression, which labels (K) satellite cells (arrowheads) and (M) Schwann cells associated with lateral line processes (arrowheads) in a control embryo, but not in panels L, N, a tfap2a^-/c^MO embryo (12 of 12 tfap2a^-/c^MO embryos). (O, P) Lateral views of 28 hpf embryos processed to reveal eya1 expression, which is detected in the lateral line migrating primordium (llmp) in panel O, a control embryo, but absent in panel P, a tfap2a^-/c^MO embryo (11 of 11 tfap2a^-/c^MO MO embryos). eya1 expression in presumed pectoral fin mesoderm (pfm) remains normal in the tfap2a^-/c^MO MO embryo (11 of 11 tfap2a^-/c^MO MO embryos). (Q, R) Lateral head views of 4 dpf embryos incubated in DASPEI, which is taken up by hair cells. In panel Q, a control embryo, DASPEI-positive hair cells in the inner ear (arrowhead) and the neuromasts (arrows) are visible. In panel R, a tfap2a^-/c^MO embryo, DASPEI positive cells are not seen. (S, T) Dorsal anterior views of 28 hpf embryos processed to reveal eya1 expression. eya1 expression in olfactory placode is apparent in panel S, a control embryo. In panel R, a tfap2a^-/c^MO embryo, a small cluster of eya1-expressing cells is present in this position (11 of 11 tfap2a^-/c^MO embryos). gV, trigeminal ganglion; allg, anterior lateral line ganglion; ac, acoustic ganglion; pllg, posterior lateral line ganglion; gAD, anterodorsal lateral line ganglion; gAV, anteroventral lateral line ganglion; gVII, facial sensory ganglion; gIX, glossopharyngeal ganglion; gX, vagus ganglion; e, eye; ot, otic vesicle. Scale bars: (A–R), 100 μm; (S, T), 50 μm.

Expression Data

Genes:	eya1 foxd3
Fish:	WT tfap2a^ts213/ts213 + MO2-tfap2c
Knockdown Reagent:	MO2-tfap2c
Anatomical Terms:	immature Schwann cell mesoderm pectoral fin bud olfactory placode perineuronal satellite cell posterior lateral line primordium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tfap2a^ts213/ts213 + MO2-tfap2c
Knockdown Reagent:	MO2-tfap2c
Observed In:	cranial ganglion cranial ganglion perineuronal satellite cell dorsal root ganglion enteric nervous system neuron hair cell lateral line system myelinating Schwann cell neuromast hair cell sympathetic nervous system neuron
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.