FIGURE

Fig. 3

ID

ZDB-FIG-071116-2

Publication

Wingert et al., 2007 - The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros

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Fig. 3

RA Signaling Is Required for Proximal Segment Fates and to Prevent the Expansion of Distal Segment Fates. (A) Expression of segment markers and mhc at 48 hpf in wild-type embryos and raldh2 morphants, and wild-type embryos that were incubated with either DMSO (control) or DEAB for indicated times during development, by double whole-mount in situ hybridization. Brackets and arrows indicate expression domains. Abbreviations are: %e (percent epiboly), s (somite stage). Dorsal views are shown, with anterior to the left, with the exception of lateral views for gata3 and aqp3 expression. (B) Summary of nephron segmentation with respect to embryo somite number for each DEAB treatment.

Expression Data

Genes:	aqp3a cdh17 gata3 slc12a1 slc12a3 slc13a1 slc20a1a stc1l wt1b
Fish:	TU TU + MO3-aldh1a2
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Knockdown Reagent:	MO3-aldh1a2
Anatomical Terms:	cloacal chamber corpuscles of Stannius podocyte posterior pronephric duct pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO3-aldh1a2
Condition:	chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagent:	MO3-aldh1a2
Observed In:	corpuscles of Stannius podocyte pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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