FIGURE

Fig. 13

ID

ZDB-FIG-071115-21

Publication

Crotwell et al., 2007 - Gene expression patterns underlying proximal-distal skeletal segmentation in late-stage zebrafish, Danio rerio

Other Figures

All Figure Page

Back to All Figure Page

Fig. 13

Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of sox9a (top panel) and sox9b (middle panel) in median fins of zebrafish (5.6-6.3 mm). Column 1 contains a 100-bp ladder with appropriately sized bands marked at left. Column 2, with 50 hours postfertilization (hpf) whole embryos (Chiang et al.,[2001b]), and the bottom panel, with constitutively expressed ef-1α (Nordnes et al.,[1994]), were used as positive controls. Columns 3-5 show presence and relative levels of amplified RNA for caudal fin rays, caudal hypural tissue and anal fin tissue, respectively. While sox9a is strongly expressed in the sampled median fin tissues, sox9b is not detected at all, although it is present in 50 hpf whole embryos, as expected (Chiang et al.,[2001b]). Column 6 shows RT-PCR results with no template added to the reaction mix (negative control).

Expression Data

Genes:	sox9a sox9b
Fish:	WT
Anatomical Terms:	anal fin caudal fin lepidotrichium hypural whole organism
Stage Range:	Long-pec to Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.