PUBLICATION

Gene expression patterns underlying proximal-distal skeletal segmentation in late-stage zebrafish, Danio rerio

Authors
Crotwell, P.L., and Mabee, P.M.
ID
ZDB-PUB-071023-13
Date
2007
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   236(11): 3111-3128 (Journal)
Registered Authors
Crotwell, Patricia, Mabee, Paula M.
Keywords
Zebrafish, median fin, skeletal augmentation, gene expression, patterning, development, late-stage
MeSH Terms
  • Animals
  • Body Patterning/genetics*
  • Cartilage/embryology
  • Cartilage/metabolism
  • Extremities/embryology
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Joints/embryology
  • Joints/metabolism
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Molecular Sequence Data
  • Morphogenesis/genetics
  • Phylogeny
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
17948314 Full text @ Dev. Dyn.
Abstract
Timing and pattern of expression of ten candidate segmentation genes or gene pairs were reviewed or examined in developing median fins of late-stage zebrafish, Danio rerio. We found a general correspondence in timing and pattern of expression between zebrafish fin radial segmentation and tetrapod joint development, suggesting that molecular mechanisms underlying radial segmentation have been conserved over 400 million years of evolution. Gene co-expression during segmentation (5.5-6.5 mm SL) is similar between tetrapods and zebrafish: bmp2b, bmp4, chordin, and gdf5 in interradial mesenchyme and ZS; bapx1, col2a1, noggin3, and sox9a in chondrocytes. Surprisingly, wnt9a is not expressed in the developing median fins, though wnt9b is detected. In contrast to all other candidate segmentation genes we examined, bapx1 is not expressed in the caudal fin, which does not segment. Together, these data suggest a scenario of gene interactions underlying radial segmentation based on the patterns and timing of candidate gene expression.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping