FIGURE

Fig. 7

ID

ZDB-FIG-070925-10

Publication

Lee et al., 2007 - Glycogen synthase kinase 3alpha and 3beta have distinct functions during cardiogenesis of zebrafish embryo

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Fig. 7

GSK3β modulates zebrafish cardiac valve formation. Whole-mount in situ hybridization with bmp4 and versican staining reveals that cardiac valve development was affected in gsk3β morphants. At 60–72 hpf,versican (A, B) and bmp4 (C, D) expression was greatly up-regulated in gsk3β morphants. Tg(cmlc2: Hc-RFP) embryos were injected with gsk3β-MO and observed by in vivo two-photon fluorescence imaging of a live transgenic zebrafish heart at 100 hpf. The endocardial cells and blood are labeled yellow; the Hc-GFP-positive myocardial cells are labeled red. Valves are clearly observed in wild-type embryos (E; white arrows), but not in gsk3β morphants (F). b, blood cells; V, ventricle; A, atrium.

Expression Data

Genes:	bmp4 vcana
Fish:	zf10Tg zf10Tg + MO1-gsk3ba
Knockdown Reagent:	MO1-gsk3ba
Anatomical Term:	heart valve
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf10Tg + MO1-gsk3ba
Knockdown Reagent:	MO1-gsk3ba
Observed In:	heart valve
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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