FIGURE

Fig. 2

ID

ZDB-FIG-070911-36

Publication

Maves et al., 2007 - Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation

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Fig. 2

Validation of microarray-identified Pbx-dependent genes. (A-Z) RNA in situ expression of genes from Table 1 in (A,C,E,G,I,K,M,O,Q,S,U,X) wild-type control, (B,D,F,H,J,L,N,P,R,T,V,Y) pbx2-MO; pbx4-MO, or (W,Z) myod-MO embryos. (S,T) Control gene, desm; (X-Z) slow-muscle gene, smyhc1. Although pbx2-MO; pbx4-MO embryos appear delayed relative to controls, all embryos are stage-matched at (A-T) 18-somite stage (18s), (U-W) 16s or (X-Z) 14s. krox-20 was included in all in situs to control for the pbx MOs. (A) r3 and r5 indicate krox-20 expression in hindbrain rhombomeres. (Q) Arrow marks vmhc expression in the heart primordium. Embryos are shown in (A-P,S-T) left-side view or (Q-R,U-Z) dorsal view, anterior towards the left.

Expression Data

Genes:	aldh1a2 atp2a1 chrna1 desma egr2b myh7 myhc4 mylpfa myod1 smyhc1 srl tmem161a ttn.1
Fish:	WIK/AB WIK/AB + MO7-myod1 + MO8-myod1 WIK/AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-pbx2 MO2-pbx4 MO3-pbx4 MO7-myod1 MO8-myod1
Anatomical Terms:	adaxial cell heart primordium immature eye lateral plate mesoderm rhombomere 3 rhombomere 5 segmental plate somite tail bud
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK/AB + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Observed In:	skeletal muscle fiber development
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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