FIGURE

Fig. 2

ID

ZDB-FIG-070821-32

Publication

Kinna et al., 2006 - Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Morpholino knockdown of zebrafish crim1. (A) Schematic showing the position of morpholino oligos designed across the start codon and (crim1A-MO) and to a region of the 5′ UTR (crim1B-MO) of zebrafish crim1 mRNA. To test the ability of crim1A-MO to knockdown expression of its target sequence, a Gfp reporter construct was produced. The start codon and first seven amino acids encode zebrafish crim1 sequence. (B) Brightfield and darkfield images showing that crim1A-MO but not a randomised control morpholino can block the production of GFP protein in zebrafish embryos injected with 500 pg of crim1-Gfp chaemeric mRNA. Scale bar=500 μm. (C) Graph showing the distribution of phenotypes observed by injection of 2.5 or 5 ng of control, crim1A-MO or crim1B-MO. Mild are embryos as represented in gray and severe in black. Standard error is deviation between separate injection experiments (n=4, 50–100 embryos/experiment).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 123(4), Kinna, G., Kolle, G., Carter, A., Key, B., Lieschke, G.J., Perkins, A., and Little, M.H., Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development, 277-287, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.