FIGURE

Fig. 6

ID

ZDB-FIG-070815-10

Publication

Auman et al., 2007 - Functional Modulation of Cardiac Form through Regionally Confined Cell Shape Changes

Other Figures

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Fig. 6

haf^sk24 Is a Strong Loss of Function Allele of vmhc, a Ventricle-Specific Myosin Heavy Chain Gene with Expression Complementary to amhc. (A and B) Fluorescent in situ hybridization for vmhc (green) and amhc (red) in wild-type (wt) embryos at LHT ([A] 26 hpf, dorsal view) and expanded chamber ([B] 48 hpf, frontal view) stages. At all stages examined, vmhc is expressed in the ventricular myocardium [51]. In contrast, amhc expression is restricted to the atrial myocardium [28]. (C) Comparison of vmhc coding sequence in wt and haf^sk24 embryos reveals a C to T transition at position 3,094 that results in a premature stop codon in haf^sk24. (D and E) Whole-mount in situ hybridization depicts frontal view of vmhc expression in wt and haf at 48 hpf. vmhc expression is dramatically reduced in the haf mutant ventricle.

Expression Data

Genes:	myh6 myh7
Fish:	WT myh7^sk24/sk24
Anatomical Terms:	atrial myocardium ventricular myocardium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myh7^sk24/sk24
Observed In:	cardiac ventricle
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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