Zebrafish cohesin functions in sister chromatid cohesion and is necessary for normal expression of Runx genes in early embryogenesis. (A-C) Mitotic defects in nz171 embryos. Confocal immunofluorescence images, stained for Rad21 (green) and α-tubulin (red), with DNA stained with DAPI (blue), as indicated on top of the panels; embryo identity is on the left. By 48 h.p.f., Rad21 protein is absent from nz171 embryos, and multiple cells have arrested in mitosis (C-C''). (D,E) Knocking down the function of Rad21 (D-D'') or Smc3 (E-E'', exon 5 splice site) reproduces the mitotic phenotype observed in nz171 embryos at 48 h.p.f. Confocal immunofluorescence images, stained for α-tubulin (red), with DNA stained with DAPI (blue), as indicated in the panels (top); embryo identity is on the left. For A-E, images are of flat-mounted embryo tails; scale bar is 30 µm. (F-I) Loss of runx1 and runx3 expression in smc3 morphants. Flat-mounted 10-somite embryos, anterior to the left. smc3 morphants (exon 2 splice site) show loss of runx1 expression in the PLM (H), and runx3 expression in the RB cells (I).
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