FIGURE

Fig. 2

ID

ZDB-FIG-070628-1

Publication

Lin et al., 2007 - Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis

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Fig. 2

Med10 is a conserved subunit of the transcriptional Mediator complex (MED). (A) Med10 is evolutionarily conserved. The predicted protein sequence for Med10 was compared using ClustalW with Med10 orthologues from several species. (B) Zebrafish Med10 is highly homologous with human and mouse Med10 but exhibits limited homology with Drosophila, C. elegans, and yeast. Shown are percentages of identity and similarity. (C) med10 is expressed throughout zebrafish embryogenesis. Shown is a Northern blot with total RNA extracted from 10 embryos at different stages. Note the decrease of maternal transcripts at the bud stage and gradual increase of zygotic transcripts thereafter.

Expression Data

Gene:	med10
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	4-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-med10 WT + MO2-med10
Knockdown Reagents:	MO1-med10 MO2-med10
Observed In:	heart pectoral fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 303(2), Lin, X., Rinaldo, L., Fazly, A.F., and Xu, X., Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis, 536-548, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.