FIGURE

Fig. 5

ID

ZDB-FIG-070620-15

Publication

Robu et al., 2007 - p53 activation by knockdown technologies

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Fig. 5

p53 MO Does Not Affect Specific Cell Death or Phenotypes Not Associated with Apoptosis (A–B) Brightfield image of chordin morphant (3ng MO) injected (A) or not (B) with p53 MO (2 ng) (1 dpf). (C–D) p53 MO does not affect the localized tail cell death, as also shown by the TUNEL assay (1 dpf). (E–F) Brightfield images of 2 dpf uninjected embryo: lateral (E) and dorsal view (F). (G–H) p53 MO (4 ng) does not affect (H) the lack of dorsal melanophores induced by nacre MO (9 ng) (G). (I–J) no tail (3 ng) phenotype (I) is not affected by coinjection of 4 ng of p53 MO (J). (K–L) UROD MO (9 ng) induces an autofluorescence of red blood cells (K). This phenotype is not affected by p53 MO coinjection (4 ng) (L) (1 dpf).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-chrd WT + MO1-chrd + MO4-tp53 WT + MO1-mitfa WT + MO1-mitfa + MO4-tp53 WT + MO1-tbxta WT + MO1-tbxta + MO4-tp53 WT + MO1-urod WT + MO1-urod + MO4-tp53
Knockdown Reagents:	MO1-chrd MO1-mitfa MO1-tbxta MO1-urod MO4-tp53
Observed In:	melanocyte nucleate erythrocyte post-vent region
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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