Fig. 6

mib expression is negatively regulated by Su(H)-dependent Notch activation. (A) mib expression in (a) wt and (b) mib^ta52b embryos at 22hpf, lateral view. The inserts show the mib expression in (a′–b′) the brain region, dorsal view and (a″–b″) a transverse section across posterior trunk. (B) her4 expression in (a–a′) control wt and (b–b′) su(h)-m7-injected embryos at 22hpf; (a–b) head region, dorsal view; (a′–b′) trunk region, lateral view. mib expression in (c) control wt and (d) su(h)-m7-injected embryos at 22hpf, lateral view. The inserts show the mib expression in the (c′–d′) head region, dorsal view and (c″–d″) a transverse section across posterior trunk. (C) her4 expression in (a–a′) control wt, (b–b′) DAPT-treated and (c–c′) X-Su(H)/Ank mRNA-injected embryos at 22hpf; (a–c) head region, dorsal view; (a′–c′) trunk region, lateral view. mib expression in (d–d′) control wt, (e–e′) DAPT-treated, and (f) X-Su(H)/Ank mRNA-injected embryos at 22hpf; (d–f) mid- and hindbrain region, dorsal view; (d′–e′) trunk and tail region, lateral view. (D) Coexpression of mib with ngn1 or dbx1a in hindbrain differentiating neurons at 42hpf. (a–c) and (e) are dorsal view; (d) and (f–h) are lateral view. Note that ngn1 and dbx1a are coexpressed with mib in different subpopulations of neurons.