FIGURE

Fig. 6

ID

ZDB-FIG-070312-9

Publication

Panizzi et al., 2007 - New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia

Other Figures

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Fig. 6

Loss of Arhgef11 function leads to formation of pronephric cysts. (A-D′) Fluorescent immunostaining of 24 hpf WT control and MO^AUG-injected embryos to visualize zRG (green), F-actin (red) with magnified view inset, and acetylated tubulin (blue). Scale bar, 20 μm. (E-G) JB-4 sections of 54 hpf WT control embryos (E) compared with sections from two embryos injected with 4 ng MO^AUG (F,G) at the same stage. Pronephric ducts are indicated by white arrowheads, and their lumens are outlined by dotted lines. Scale bar, 25 μm. (H,I) Live embryos at 80 hpf showing an uninjected WT embryo (H) compared with WT injected with 4 ng MO^AUG (I), which exhibits cardiac edema (arrowhead) and pronephric cysts (arrow). Scale bar: 300 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-arhgef11
Knockdown Reagent:	MO1-arhgef11
Observed In:	pericardium pronephric duct pronephric duct actin cytoskeleton pronephric duct cilium
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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