FIGURE

Fig. 2

ID

ZDB-FIG-070228-2

Publication

Feng et al., 2006 - Hedgehog acts directly on the zebrafish dermomyotome to promote myogenic differentiation

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Fig. 2

Hedgehog signaling negatively regulates the number of Pax7⁺ myogenic precursors. Embryos were labeled at the prim5 (24 h) stage for Pax7 (green) and myosin (MF20, red). (A) In wild-type embryos, the surface of each somite has two types of Pax7⁺ myogenic precursors, moderately labeled cells that are part of the somite, and more brightly labeled, dorso-ventrally elongated neural crest cell nuclei. (B, C) smu(smo)^-/- and CyA-treated wild-type embryos have more Pax7⁺ myogenic precursors than wild-type control embryos, particularly in the anterior part of each somite (arrows). (D, E) In syu(shh)^-/- and yot(gli2)^-/- embryos, the number of Pax7⁺ nuclei is intermediate between untreated wild-type and smu(smo)^-/- or CyA-treated wild-type embryos. (F) shh mRNA-injected wild-type embryos have virtually no Pax7⁺ nuclei. (G) A single optical slice of a CyA-treated wild-type embryo through the middle part of somites, demonstrating the gaps between myotomes that are filled with Pax7⁺ cells. Many of the Pax7⁺ cells are within the anterior of each somite (dotted outline). (H) Quantitation of the number of Pax7⁺ myogenic precursors per somite in each genotype or treatment (mean of 5 embryos per genotype or treatment, somites 16–18 were counted; error bar = standard deviation). Panels A–F are projections of Z-stack confocal sections (see Materials and methods), panel G is a thin optical slice, all panels are anterior to the left, dorsal up; somite 17 is shown in each panel. Scale bar = 50 μm.

Expression Data

Gene:	pax7a
Fish:	AB gli2a^ty119/ty119 (AB) shha^{tbx392/tbx392} (AB) smo^b641/b641 (AB)
Condition:	chemical treatment: Cyclopamine
Anatomical Terms:	dermomyotome neural crest cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli2a^ty119/ty119 (AB) shha^{tbx392/tbx392} (AB) smo^b641/b641 (AB)
Observed In:	muscle cell differentiation muscle precursor cell somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 300(2), Feng, X., Adiarte, E.G., and Devoto, S.H., Hedgehog acts directly on the zebrafish dermomyotome to promote myogenic differentiation, 736-746, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.