FIGURE

Fig. 1

ID

ZDB-FIG-070116-1

Publication

Liao et al., 2000 - Hereditary spherocytosis in zebrafish riesling illustrates evolution of erythroid ß-spectrin structure, and function in red cell morphogenesis and membrane stability

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Embryonic blood defects in ris. In this and all subsequent figures embryos are shown as lateral views, with anterior left and ventral down, at 10X magnification, unless specified otherwise. (A) Whole-mount o-dianisidine staining of wildtype and ris embryos at 4 dpf, showing absence of blood cells in ris (arrowhead). (B) Wright- Giemsa staining of embryonic blood cells collected from wild-type and ris embryos at 2.5 dpf. Wild-type primitive erythrocytes are spherical and nucleated, whereas ris primitive erythrocytes are often abnormally shaped (arrowhead) and binucleated (asterisk). 20X magnification. (C) In situ analysis of ris embryos at 23 hours post-fertilization (hpf), with antisense riboprobes of scl, gata-1 and alas-e. At 23 hpf, blood cells are restricted to the intermediate cells mass (ICM), dorsal to the yolk tube extension (arrow).

Expression Data

Genes:	alas2 gata1a tal1
Fish:	sptb^tb237/tb237
Anatomical Term:	intermediate cell mass of mesoderm
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sptb^tb237/tb237
Observed In:	nucleate erythrocyte
Stage Range:	Pec-fin to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development