Fig. S1

Migratory pigment cells recover in the mos^m188 mutant embryos. Migratory pigment precursors in wild type (A, C, E, G, I) lateral and (A?, C?, E?, G?, I?) dorsal views, and in mos^m188 mutants (B, D, F, H, J) lateral and (B?, D?, F?, H?, J?) dorsal views as revealed by whole mount in situ hybridization of 30 hpf embryos. Although expression patterns in mos^m188 mutants lack behind their wild-type siblings, the differences are less pronounced than at 22 hpf in Fig. 4. (A-B?) Expression of mitfa, the early marker of pigment progenitors is present in the most distally and ventrally located cells in wild types and in mos mutants, but in mos^m188 expression is reduced in the anteroposterior and dorsoventral extent. (C-D?) The expression of the mitfa target gene kit is only slightly reduced in mos^m188 mutants as the migrating cells begin to spread over the yolk (C?, D?). (E-F?) In contrast, expression of the xanthine dehydrogenase gene in lineage-committed xanthoblasts still lacks behind in mos^m188 mutants. (G-H?) A similar defect is evident in expression of the GTP-cyclohydrolase, a critical enzyme in the pteridine and melanine synthesis. (I-J?) The dct/trp2 gene that is largely absent in mos at 22 hpf (Fig. 4) is extensively expressed in migratory precursors in the mutant embryos at 30 hpf.