FIGURE

Fig. 3

ID

ZDB-FIG-061122-10

Publication

Kwak et al., 2006 - Zebrafish pax5 regulates development of the utricular macula and vestibular function

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Fig. 3

cDNA structure and expression of pax5. A: General structure of pax5 splice variants. Brackets indicate exon boundaries. Conserved functional domains, paired (PD), octapeptide (OP), homeo (HD), transactivation (TAD), and inhibitory (ID) are marked. Putative translation start sites (M) are indicated. Binding sites for translation-blocking (TB1 and 2) and splice-blocking (SB1, 2, and 3) morpholinos are shown. Newly identified 5′ (i) and 3′ (ii) sequences are shown in comparison with fugu pax5. Zebrafish and fugu sequences are 100% identical at the amino acid level. B-E: Expression of pax5 in the otic placode at 17 hours postfertilization (hpf; B), in the otic vesicle at 24 hpf (C) and in the utricular macula at 48 hpf (D,E). E: Enlarged view of boxed area in D. Hair cell (hc) supporting cell layers are marked. Arrow, weak expression in the saccule. (A) Dorsal, (B) dorsolateral, and (C,D) lateral views, with anterior to the left. Scale bar = 30 μm in A, 40 μm in B,C, 12.5 μm in D.

Expression Data

Genes:	dlx3b egr2b fgf3 fgf8a hmx3a msx3 otx1 pou3f3b
Fish:	AB AB + MO1-pax5 + MO2-pax5 + MO3-pax5 + MO4-pax5 + MO5-pax5
Knockdown Reagents:	MO1-pax5 MO2-pax5 MO3-pax5 MO4-pax5 MO5-pax5
Anatomical Terms:	anterior crista primordium lateral crista primordium midbrain hindbrain boundary neural rod otic vesicle posterior crista primordium presumptive rhombomere 3 presumptive rhombomere 5
Stage Range:	5-9 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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