FIGURE

Fig. 5

ID

ZDB-FIG-061121-32

Publication

Kopinke et al., 2006 - Retinoic acid is required for endodermal pouch morphogenesis and not for pharyngeal endoderm specification

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Fig. 5

RA is required during morphogenesis of the posterior endodermal pouches. Anterior endodermal pouches develop earlier than more posterior ones and inhibition of RA at different time points only affects newly forming pouches. A-D: ZN8 immunostaining of endodermal pouches, schematic drawing of pouches in 30-hpf control embryos, as well as cartilage staining of 96-hpf embryos. A: 30-hpf control embryos possess five endodermal pouches. At 96 hpf, 7 pharyngeal cartilages have formed. B: Embryos treated with DEAB between 16-30 hpf possess three pouches, pouches 4-5 are absent. At 96 hpf, 3 pharyngeal cartilages have formed. C: Embryos that were treated with DEAB between 20-30 hpf possess three fully formed pouches, the fourth one is only half formed and pouch 5 is missing. At 96 hpf, four pharyngeal cartilages have formed. D: Embryos that were treated between 24-30 hpf have four endodermal pouches and the fifth one is missing. At 96 hpf, five pharyngeal cartilages have formed. Abbreviations are the same as in Figure 3.

Expression Data

Gene:	alcama
Fish:	WT
Condition:	chemical treatment: 4-(diethylamino)benzaldehyde
Anatomical Terms:	pharyngeal pouch 1 pharyngeal pouch 2 pharyngeal pouch 3 pharyngeal pouch 4 pharyngeal pouch 5
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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