FIGURE

Fig. 5

ID

ZDB-FIG-061104-27

Publication

Berndt et al., 2006 - Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain

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Fig. 5

ΔTCF^gfp disrupts cell cycle and crestin expression and eliminates cyclin D1 and sema3d expression. (A-I) Immunodetection of BrdU (A,B,I) and in situ hybridization for crestin (C,D), cyclinD1 (E,F), sema3d (G,H) in the hindbrain at 17 hpf of heat-shocked wild-type siblings (A,C,E,G), ΔTCF^gfp transgenic (B,D,F,H), and ΔTCF^gfp + Sema3d^myc double-transgenic (I) embryos. (J) Quantification of the number of BrdU-labeled nuclei in the neuroepithelium of rhombomeres 3-5. Numbers in parentheses indicate n. ^*P<10^-13 versus wild-type sibling, #P<10^-4 versus ΔTCF^gfp, t-test. (A-I) Dorsal views, anterior is leftwards. oto, otocyst. Scale bar: 40 μm for A-I.

Expression Data

Genes:	ccnd1 crestin sema3d
Fish:	WT w26Tg (AB)
Condition:	heat shock
Anatomical Terms:	hindbrain neural crest cell rhombomere
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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