Fig. 4

 Neuronal and pancreatic expression of insm1a (A, C, E, G, and I) and insm1b (B, D, F, H, and J) in zebrafish. All panels show anterior to the left with complete (A–D), lateral (E and F), and ventral focal planes (G–J). Scale bars represent 50 μm. Fluorescein labeled elavl3 (A–F) or neuroD (G–J) antisense probe was used to indicate specified neurons or pancreatic cells (red), respectively. insm1 is shown in purple and the resulting overlap appears brown with varying intensities. At 11 hpf (A and B) elavl3 is expressed in neurons of the early CNS and insm1 overlap this expression precisely in several types of neurons. At 24 hpf (C and D) there is a large degree of overlap between insm1 and elavl3. By 48 hpf (E and F) elavl3 labels the entire CNS and darker regions indicate where insm1 is expressed, notably the cranial ganglia for insm1b and retina for insm1a. As a reference for elavl3-only (red) neurons, insm1a is not present in pmn at 11 hpf and barely detectable in the cranial ganglia at 24 hpf; otic vesicles are indicated for positional reference. Pancreatic expression of insm1a and insm1b coincide with neuroD in a disorganized manner at 18 hpf (G and H) and later come together to a continuous expression domain at 24 hpf (I and J). cb, cerebellum; cg, cranial ganglia; in, interneurons; mb, midbrain; nc, notochord; olf, olfactory placode; ot, otic vesicle; pmn, primary motoneurons; Rb, Rohon-beard neurons; ret, retina; tel, telencephalon; and tg, trigeminal ganglia.