FIGURE

Fig. 2

ID

ZDB-FIG-060918-1

Publication

Lecaudey et al., 2004 - The zebrafish Iroquois gene iro7 positions the r4/r5 boundary and controls neurogenesis in the rostral hindbrain

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Fig. 2

Blocking iro7 translation with Moz7 leads to a reduction of the midbrain and anterior hindbrain. (A) Autoradiography showing radiolabelled in vitro translation products of the iro7 capped RNA in the presence of increasing concentrations of Moz7 or Moz7m (0.4 μM to 80 μM). (B,C) Lateral view of live embryos at 30 hpf injected with Moz7 (B) or Moz7m (C), anterior is towards the left. (D-O) Embryos stained by in situ hybridisation with the indicated probes (colour coded). Anterior is towards the left, stages are indicated at the bottom right-hand corner of each picture. (B-G,J,K,N,O) Whole mounts; (H,I,L,M) flat-mounts. is, isthmus; ov, otic vesicle; os, optic stalk; MHB, midbrain-hindbrain boundary; ep, epiphysis.

Expression Data

Genes:	egr2b fgf3 fgf8a hoxa2b pax2a wnt1
Fish:	WT WT + MO2-irx7
Knockdown Reagent:	MO2-irx7
Anatomical Terms:	epiphysis midbrain hindbrain boundary neural rod neural plate neural rod otic placode presumptive brain presumptive rhombomere 2 presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5 rhombomere 3 rhombomere 5
Stage Range:	75%-epiboly to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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