FIGURE

Fig. 4

ID

ZDB-FIG-060522-49

Publication

Foucher et al., 2006 - Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

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Fig. 4

Formation of the granule cell population in fgf8^-/-; OtxH embryos. atoh1a expression in wild-type (A), OtxH (B), fgf8^-/- (C) and fgf8^-/-; OtxH (D) whole-mount embryos. MyoD expression in the somite was concomitantly detected to confirm fgf8^-/-genotype. (E-O) Para-saggital 10 μm sections, anterior towards the left, of wild-type her5pac:egfp (E,F), OtxH (H,I), fgf8^-/- (K,L) and fgf8^-/-; OtxH (N,O) brains, showing co-localisation of atoh1a (E,H,K,N) and GFP expression (F,I,L,O). Granule cells absent in fgf8^-/- are rescued in fgf8^-/-; OtxH and are GFP positive (white lines on fluorescent pictures represent the length of the I>atoh1a domain in the upper rhombic lip obtained from bright field pictures). (G,J,M,P) Cartoons summarising GFP (in green) and atoh1a (in blue) expressions in wild-type (G), OtxH (J), fgf8^-/- (M) and fgf8^-/-; OtxH (P) embryos carrying the transgene her5pac:egfp.

Expression Data

Gene:	atoh1a
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO1-otx2a + MO1-otx2b fgf8a^{ti282a/ti282a} + MO1-otx2a + MO1-otx2b
Knockdown Reagents:	MO1-otx2a MO1-otx2b
Anatomical Term:	midbrain hindbrain boundary
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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