FIGURE

Fig. 2

ID

ZDB-FIG-051101-5

Publication

Groves et al., 2005 - Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafish

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Fig. 2

Fgf8 is required for fast muscle differentiation in the lateral somite. (A) Cryostat sections at the gut extension level of whole-mount in situ mRNA hybridisation for myosin light chain 2 (mylz2) at 20-22 s in unmanipulated control, Fgf8 MO-treated, ace mutant and SU5402-treated embryos. Red brackets show expansion of the lateral undifferentiated tissue. (B-D) Immunohistochemical detection of fast MyHC (B) or slow MyHC (C,D) on whole mounts at 15 s (C) or cryosections of Fgf8 MO-injected and control embryos at 33 hpf (B,D). Brackets indicate expansion of lateral non-muscle tissue, arrows show weakened fast MyHC.

Expression Data

Gene:	mylpfa
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO3-fgf8a
Condition:	chemical treatment: SU5402
Knockdown Reagent:	MO3-fgf8a
Anatomical Term:	somite
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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