FIGURE

Fig. 3

ID

ZDB-FIG-050923-3

Publication

Fong et al., 2005 - Wnt signalling mediated by Tbx2b regulates cell migration during formation of the neural plate

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Fig. 3

`Exclusion' phenotype can be rescued by tbx2b mRNA. Compared with control (A), a 1/16 injection of tbx2b MO led to `exclusion' phenotype (B) at 10 hpf. Neural plate highlighted with sox19 in blue; fluorescein labelled cells in brown. By contrast, 1/16 injection of tbx2b mRNA (1.0 ng) led to (C) labelled cells with normal morphology in the midline (arrow) at 10 hpf and, (D) the notochord (arrows) at 24 hpf, consistent with the role of Tbx2b in axial mesodermal specification. (E) Exclusion phenotype at 10 hpf from tbx2b MO (0.05 pmol) was rescued with tbx2b mRNA lacking the target site in a dose-dependent manner. In the presence of tbx2b MO, 1.0 ng mRNA led to appearance of labelled cells in the midline similar to C and D, while significantly reducing the number of embryos with `exclusion' phenotype. 0.5 ng mRNA was less effective in reducing the `exclusion' phenotype but did not cause appearance of labelled cells in the midline.

Expression Data

Gene:	sox19a
Fish:	WT WT + MO3-tbx2b
Knockdown Reagent:	MO3-tbx2b
Anatomical Term:	unspecified
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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