FIGURE

Fig. 5

ID

ZDB-FIG-050824-8

Publication

O'Hara et al., 2005 - Zebrafish Lmx1b.1 and Lmx1b.2 are required for maintenance of the isthmic organizer

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Following knockdown of Lmx1b.1 and Lmx1b.2, IsO genes are initiated normally, but some are not maintained. Lateral views of anterior CNS, with dorsal upwards and anterior leftwards. Embryos were injected with B1B2-MO (A,C,E,G) or BX-MO (B,D,F,H). wnt1 is initiated normally at the 10-somite stage. (A,B), but fades at the MMR by 15-18 somites (C,D). pax8 expression fades by 15-18 somites (E,F), and fgf8 fades by 19-22 somites, with partial dorsal retention (G,H). Red arrowheads indicate the MMR.

Expression Data

Genes:	fgf8a pax8 wnt1
Fish:	TL + MO1-lmx1ba + MO1-lmx1bb
Knockdown Reagents:	MO1-lmx1ba MO1-lmx1bb
Anatomical Terms:	midbrain hindbrain boundary midbrain hindbrain boundary neural rod
Stage Range:	10-13 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development