Fig. 4
- ID
- ZDB-FIG-050527-1
- Publication
- Londin et al., 2005 - Chordin, FGF signaling, and mesodermal factors cooperate in zebrafish neural induction
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Recovery of anterior neural tissue following FGF inhibition depends on Nodal signaling. (A–H) otx2 in situ hybridization of wild type, MZoep, and MZoep; dino embryos microinjected with 125 pg XFD or GFP mRNA. Expression of otx2 is comparable at the six-somite stage in wild-type embryo microinjected with GFP or XFD mRNA (A and B), while XFD-injected MZoep embryos have a reduced otx2 domain (C and D). The otx2 domain is comparable in XFD- and GFP-injected MZoep;dino mutant embryos at the six-somite stage (E and F), while otx2 expression is eliminated in XFD-injected MZoep; dino embryos at 70% epiboly (mid-gastrula) (G and H). Stages of embryos are indicated. Panels A–F are lateral views, G and H are dorsal views. (I) Real-time PCR analysis of otx2 transcript levels in wild type and MZoep XFD-injected embryos at the six-somite stage. The fold change (y-axis) of these markers is set relative to control (GFP)-injected embryos. XFD-injected MZoep embryos show a decrease in otx2 expression while XFD-injected wild-type embryos show no change in otx2 expression compared to controls. |
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| Stage: | 5-9 somites |
Reprinted from Developmental Biology, 279(1), Londin, E.R., Niemiec, J., and Sirotkin, H.I., Chordin, FGF signaling, and mesodermal factors cooperate in zebrafish neural induction, 1-19, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.