Fig. 1

Neural tissue is maintained in MZoep;dino double mutants. Lateral views of living MZoep and MZoep;dino double mutants (A and B) at 22 h. The double mutant has reduced neural tissue and large tail somites compared to MZoep single mutants. Analysis of the expression patterns of neural and presumptive epidermal markers in MZoep and MZoep;dino double mutants by whole mount RNA in situ hybridization. The presence of neural tissue at 22 h is confirmed by the expression of emx1 and krox20 (C and D). The expression domains of the anterior neural markers otx2 and opl and the posterior neural marker hoxb1b are reduced in MZoep;dino double mutants (F, H, and J) compared to MZoep mutants (E, G, and I). The gata2 expression domain is expanded dorsally in MZoep;dino double mutants (K and L). During early somitogenesis, the neural plate is narrow in the double mutant as revealed by krox20 and pax2.1 expression (M–P). Panels C and D are lateral views; E–H are dorsal views at 70% epiboly (mid-gastrula); I and J are dorsal views at late gastrula (90% epiboly); K and L are animal pole views at 70% epiboly (mid-gastrula); and M–P are dorsal views at the three-somite stage. Genotypes of all embryos were determined following photography by PCR-based analysis.