Fig. 2

Phenotypes caused by reducing Bmp signaling at various developmental stages. Embryos from a transgenic outcross were heatshocked in an air incubator at 3 hpf (A-C) or shield stage (D-F), and the GFP-positive embryos were sorted and scored for dorsalized phenotypes at 30 hpf. Note the fully formed tail in the wild-type sibling (whole embryo in A, tail view in D). Transgenic embryos heatshocked at 3 hpf display both C4 (B) and C3 dorsalized (C) phenotypes. Note the curled trunk and tail in B and the curled tail in C. Transgenic embryos heatshocked at shield stage display either a C1 phenotype (E; arrow denotes loss of the ventral tail fin) or a C1 phenotype coupled with the formation of an ectopic tail (F; arrowhead denotes second tail). ET, ectopic tail.