FIGURE

Fig. 5

ID

ZDB-FIG-050425-5

Publication

Essner et al., 2005 - Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut

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Fig. 5

ntl and the Nodal signaling pathway regulate lrdr1 expression, and, with other genes, control morphogenesis of Kupffer′s vesicle. Wild-type (A,F) and mutant ntl^b195(B,G), spt^b104(C,H), cas^ta56 (D,I) and oep^m134 (E,J) embryos were examined for lrdr1 mRNA expression, cilia formation and KV organization. (A-E) lrdr1 expression at 80-90% epiboly. Lateral views, dorsal towards the right, anterior upwards; arrows indicate DFCs. (F-J) Immunofluorescence analysis of cilia and KV morphogenesis using anti-acetylated Tubulin antibodies (cilia, green) and anti-Ntl antibodies (KV, red) at 6-10 SS. Dorsal views with anterior towards the left. Arrows indicate cilia.

Expression Data

Genes:	dnah9 tbxta
Fish:	WT sox32^ta56/ta56 tbx16^b104/b104 (AB) tbxta^b195/b195 tdgf1^m134/m134 (AB)
Anatomical Terms:	forerunner cell group Kupffer's vesicle notochord
Stage Range:	75%-epiboly to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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