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Fig. 5 ntl and the Nodal signaling pathway regulate lrdr1 expression, and, with other genes, control morphogenesis of Kupffer′s vesicle. Wild-type (A,F) and mutant ntlb195(B,G), sptb104(C,H), casta56 (D,I) and oepm134 (E,J) embryos were examined for lrdr1 mRNA expression, cilia formation and KV organization. (A-E) lrdr1 expression at 80-90% epiboly. Lateral views, dorsal towards the right, anterior upwards; arrows indicate DFCs. (F-J) Immunofluorescence analysis of cilia and KV morphogenesis using anti-acetylated Tubulin antibodies (cilia, green) and anti-Ntl antibodies (KV, red) at 6-10 SS. Dorsal views with anterior towards the left. Arrows indicate cilia.