Fig. 3

Wild-type, sox9 mutants, and sox9b-morpholino-treated animals. (A-E) Live larvae; (F-J) lateral view of head of Alcian-stained larvae. (K-O) Flat mount of Alcian-stained pharyngeal arches. (P-T) Flat mount of Alcian-stained neurocranium. (U-Y) Alcian-stained pectoral fin bud. All animals at 4 dpf. (A,F,K,P,U) Wild type; (B,G,L,Q,V) sox9a mutant, showing deletion of most cranial cartilage and scapulocoracoid; (C,H,M,R,W) sox9b^b971; neurocranium, arches, and endochondral disc reduced; (D,I,N,S,X) sox9b MO-injected animals phenotypically similar to sox9b^b971; (E,J,O,T,Y) double mutant, arches, neurocranium, and fin deleted. Abbreviations: abc, anterior basicranial commissure; cbs, ceratobranchials; ch, ceratohyal, m, Meckel's cartilage; cl, cleithrum; co, scapulocoracoid; ed, endochondral disc; ep, ethmoid plate; hs, hyosymplectic; j, jaw; ov, otic vesicles; no, notochord; pc, parachordal; pq, palatoquadrate; tr, trabecula. Scale bars: in E, 100 µm for A-E; in T, 100 µm for F-T; in Y, 100 µm for U-Y.