FIGURE

Fig. 5

ID

ZDB-FIG-050314-10

Publication

Crosnier et al., 2005 - Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine

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Fig. 5

Intestinal phenotype of mib ^ta52b homozygotes. (A,B) 2F11 staining in the intestinal bulb, comparing three representative pairs of wild-type (A) and mib ^ta52b (B) specimens at 3 days. The vast majority of epithelial cells are switched to a secretory fate in mib ^ta52b. (C,D) At the same time, the number of cells expressing DeltaD (zdd-2 antibody, green, indicated by arrows in C) is greatly increased in the mutant. Membrane localization of DeltaD in mib ^ta52b reflects the failure of Mib-dependent Delta internalization. (E,F) 4E8 staining (green) shows deficit of absorptive cells in three-day mib^ta52b. (G,H) 2H9 staining (green) shows abnormal epithelial cell polarity in mib ^ta52b. (I,J) Staining of the basement membrane with 5F11 (green) is also fainter in mib ^ta52b than in wild-type. (K,L) At 4 days, many dying cells are seen in mib ^ta52b (L) but not in wild type (K). (A-L) TOPRO-3 nuclear stain is red.

Expression Data

Gene:	dld
Fish:	WT mib1^ta52b/ta52b
Anatomical Term:	gut
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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