CRISPR

CRISPR1-sgcd

ID

ZDB-CRISPR-240806-1

Name

CRISPR1-sgcd

Previous Names

None

Target

sgcd (1)

Sequence

5' - GAGTGGGGATCTACGGCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ia401	sgcd

Expression

Gene expression in Wild Types + CRISPR1-sgcd

No data available

Phenotype

Phenotype resulting from CRISPR1-sgcd

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sgcd

Phenotype	Fish	Conditions	Figures
skeletal muscle cell morphology, abnormal	sgcd^ia401/ia401	standard conditions	Figure 4 from Dalla Barba et al., 2023
skeletal muscle cell fragmented, abnormal	sgcd^ia401/ia401	standard conditions	Figure 5 from Dalla Barba et al., 2023
whole organism Ab1-sgcd labeling absent, abnormal	sgcd^ia401/ia401	standard conditions	Figure 1 from Dalla Barba et al., 2023
skeletal muscle cell degenerate, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 from Dalla Barba et al., 2023
leukocyte Ab8-lcp1 labeling increased distribution, abnormal	sgcd^ia401/ia401	standard conditions	Figure 4 from Dalla Barba et al., 2023
swimming behavior decreased process quality, abnormal	sgcd^ia401/ia401	control	Figure 6 from Dalla Barba et al., 2023
skeletal muscle inflamed, abnormal	sgcd^ia401/ia401	standard conditions	Figure 4 from Dalla Barba et al., 2023
skeletal muscle cell sarcomere organization quality, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 , Figure 5 from Dalla Barba et al., 2023
skeletal muscle cell myoseptum detached from skeletal muscle cell myoseptum, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 from Dalla Barba et al., 2023
skeletal muscle cell mitochondrion degenerate, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 from Dalla Barba et al., 2023
skeletal muscle adipose tissue infiltrative, abnormal	sgcd^ia401/ia401	standard conditions	Figure 4 from Dalla Barba et al., 2023
skeletal muscle cell sarcoplasmic reticulum dilated, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 , Figure 5 from Dalla Barba et al., 2023
startle response decreased process quality, abnormal	sgcd^ia401/ia401	lighting conditions	Figure 2 from Dalla Barba et al., 2023
skeletal muscle cell disorganized, abnormal	sgcd^ia401/ia401	chemical treatment by environment: methyl cellulose	Figure 7 from Dalla Barba et al., 2023
muscle cell damaged, abnormal	sgcd^ia401/ia401	standard conditions	Figure 3 , Figure 5 from Dalla Barba et al., 2023
skeletal muscle cell disorganized, abnormal	sgcd^ia401/ia401	standard conditions	Figure 4 , Figure 5 from Dalla Barba et al., 2023
myotome undulate, abnormal	sgcd^ia401/ia401	chemical treatment by environment: methyl cellulose	Figure 7 from Dalla Barba et al., 2023
skeletal muscle cell sarcomere organization quality, abnormal	sgcd^ia401/ia401	chemical treatment by environment: methyl cellulose	Figure 7 from Dalla Barba et al., 2023
skeletal muscle cell myoseptum detached from skeletal muscle cell myoseptum, abnormal	sgcd^ia401/ia401	chemical treatment by environment: methyl cellulose	Figure 7 from Dalla Barba et al., 2023

Citations