CRISPR

CRISPR1-sirt2

ID

ZDB-CRISPR-230613-2

Name

CRISPR1-sirt2

Previous Names

None

Target

sirt2 (1)

Sequence

5' - AGTCTTGGATGAGTTAACCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sh626	sirt2

Expression

Gene expression in Wild Types + CRISPR1-sirt2

No data available

Phenotype

Phenotype resulting from CRISPR1-sirt2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sirt2

Phenotype	Fish	Conditions	Figures
lateral crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
anterior crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
posterior crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
posterior crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
eye ab1-tuba labeling increased amount, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 2 from Łysyganicz et al., 2021
macula kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
macula kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
lateral crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
anterior crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
inner ear cytoplasmic microtubule Ab15-tuba labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
lateral crista kinocilium ab2-tub-glyc labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
retina photoreceptor connecting cilium ab2-tub-glyc labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
posterior crista kinocilium ab2-tub-glyc labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
lateral crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021
posterior crista kinocilium ab2-tub-glyc labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
whole organism hdac6 expression increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 1 from Łysyganicz et al., 2021
anterior crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021
whole organism sirt2 expression decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 1 from Łysyganicz et al., 2021
lateral crista kinocilium ab2-tub-glyc labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
posterior crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021
eye ab1-tuba labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 2 from Łysyganicz et al., 2021
macula kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
anterior crista kinocilium ab2-tub-glyc labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
whole organism swimming behavior decreased process quality, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	mechanical stress	FIGURE 7 from Łysyganicz et al., 2021
retina cilium ab2-tub-glyc labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
retina cell body Ab15-tuba labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
retina photoreceptor connecting cilium Ab15-tuba labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
posterior crista kinocilium Ab15-tuba labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021
retina cell body Ab15-tuba labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
retina cilium ab2-tub-glyc labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
anterior crista kinocilium ab2-tub-glyc labeling increased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 5 from Łysyganicz et al., 2021
macula kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
lateral crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021
retina photoreceptor connecting cilium Ab15-tuba labeling decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
retina photoreceptor connecting cilium ab2-tub-glyc labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
whole organism hdac10 expression decreased amount, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 1 from Łysyganicz et al., 2021
inner ear cytoplasmic microtubule Ab15-tuba labeling increased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 from Łysyganicz et al., 2021
anterior crista kinocilium Ab15-tuba labeling decreased distribution, abnormal	hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626	standard conditions	FIGURE 3 , FIGURE 5 from Łysyganicz et al., 2021

Citations