CRISPR

CRISPR1-tulp1b

ID

ZDB-CRISPR-221207-3

Name

CRISPR1-tulp1b

Previous Names

None

Target

tulp1b (1)

Sequence

5' - GCCTCCATGTTTCAGACCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hzu19	tulp1b

Expression

Gene expression in Wild Types + CRISPR1-tulp1b

No data available

Phenotype

Phenotype resulting from CRISPR1-tulp1b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-tulp1b

Phenotype	Fish	Conditions	Figures
eye ab1-gnat1 labeling absent, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. S4 from Jia et al., 2022
head tulp1b expression decreased amount, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
photoreceptor outer segment layer retinal rod cell decreased length, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. S4 from Jia et al., 2022
eye ab2-gnat2 labeling decreased amount, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. S4 from Jia et al., 2022
retinal outer nuclear layer decreased thickness, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. S4 from Jia et al., 2022
photoreceptor outer segment layer decreased height, abnormal	tulp1b^hzu19/hzu19	standard conditions	Fig. S4 from Jia et al., 2022
eye ab2-gnat2 labeling absent, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
head tekt2 expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 4 from Jia et al., 2022
head tulp1a expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
head fthl28 expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head ptgs2b expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
retinal rod cell ab5-rho labeling decreased distribution, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
eye ab1-gnat1 labeling absent, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
eye photoreceptor cell ferroptosis increased process quality, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
retinal photoreceptor layer atrophied, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
retina tulp1a expression decreased distribution, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
eye Ab1-tulp1 labeling absent, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
retina cilium decreased length, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 3 from Jia et al., 2022
eye photoreceptor cell intracellular iron ion homeostasis disrupted, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head tulp1b expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
eye photoreceptor cell mitochondrial membrane decreased size, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head cyp2p10 expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head arl3l2 expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 4 from Jia et al., 2022
long single cone cell Ab2-opn1sw2 labeling decreased distribution, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
retina tulp1b expression decreased distribution, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 1 from Jia et al., 2022
head alox5b.3 expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head ab2-gnat2 labeling absent, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
head cep126 expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 4 from Jia et al., 2022
eye photoreceptor cell mitochondrial crista absence of anatomical entity, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head ab1-gnat1 labeling absent, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
retina photoreceptor disc membrane disorganized, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
head cp expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
retina accumulation retina lipid, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head gnat1 expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
eye ab2-gnat2 labeling decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. S3 from Jia et al., 2022
eye photoreceptor cell iron cation increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022
head gnat2 expression decreased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 2 from Jia et al., 2022
head cyp2p9 expression increased amount, abnormal	tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19	standard conditions	Fig. 5 from Jia et al., 2022

Citations