CRISPR

CRISPR1-pax3a

ID

ZDB-CRISPR-220203-1

Name

CRISPR1-pax3a

Previous Names

None

Target

pax3a (1)

Sequence

5' - GGTAGTTTTGGGGTGGCGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
umu5	pax3a

Expression

Gene expression in Wild Types + CRISPR1-pax3a

No data available

Phenotype

Phenotype resulting from CRISPR1-pax3a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pax3a

Phenotype	Fish	Conditions	Figures
integument pigmentation decreased process quality, abnormal	pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
whole organism pak2a expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
integument pigmentation decreased process quality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
whole organism rac1l expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism pax7b expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 7 from Nord et al., 2021
whole organism cdc42l2 expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism mapk10 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism akt3b expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism rac3b expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
nervous system process decreased process quality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 1 from Kahsay et al., 2022
whole organism ralbb expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism rac2 expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism pdpk1a expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism mapk12b expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism pak1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
adrenergic receptor signaling pathway decreased occurrence, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 1 from Kahsay et al., 2022
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
whole organism rps6ka1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism stat1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism rgl1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
whole organism rhoca expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism mapk8a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism rps6ka3a expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism akt1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
whole organism stat3 expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
ribosome biogenesis increased occurrence, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 1 from Kahsay et al., 2022
whole organism pax7a expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 7 from Nord et al., 2021
cardiac muscle cell contraction decreased occurrence, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 1 from Kahsay et al., 2022
whole organism gsk3ba expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Figure 2 from Kahsay et al., 2022
regulation of response to tumor cell decreased process quality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 3 from Kahsay et al., 2022
whole organism vegfaa expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism lamc2 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism itga6b expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism akt3b expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism rac3b expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism pak1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
regulation of MAPK cascade decreased process quality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism nfatc1 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
whole organism mapk10 expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; i150Tg/i150Tg	standard conditions	Figure 4 from Kahsay et al., 2022
integument pigmentation decreased process quality, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
trunk curved ventral, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature cell population proliferation increased occurrence, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 4 from Nord et al., 2021
integument pigmentation decreased process quality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
posterior hypaxial muscle myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 3 , Fig. 4 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 4 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
trunk curved ventral, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
myotome EGFP expression increased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4; i150Tg	perforation: somite 10	Fig. 8 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor lacks all parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
trunk curved ventral, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature paralysed, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
posterior hypaxial muscle myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021

Citations