CRISPR

CRISPR1-bud13

ID
ZDB-CRISPR-190220-1
Name
CRISPR1-bud13
Previous Names
None
Target
Sequence
5' - GGAGAGCGTGTCCTCCGTGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ya103 bud13
Expression
Gene expression in Wild Types + CRISPR1-bud13
No data available
Phenotype
Phenotype resulting from CRISPR1-bud13
No data available
Phenotype of all Fish created by or utilizing CRISPR1-bud13
Phenotype Fish Conditions Figures
brain morphology, abnormal bud13ya103/ya103 standard conditions Fig. 1 with image from Fernandez et al., 2018
mRNA splicing, via spliceosome process efficacy, abnormal bud13ya103/ya103 standard conditions Fig. 3 from Fernandez et al., 2018
mRNA splice site recognition disrupted, abnormal bud13ya103/ya103 standard conditions Fig. 3 from Fernandez et al., 2018
central nervous system cell death increased occurrence, abnormal bud13ya103/ya103 standard conditions Fig. 1 with image,  Fig. 2 with image from Fernandez et al., 2018
glutamatergic neuron DsRed expression decreased amount, abnormal bud13ya103/ya103; nns9Tg; ot1Tg standard conditions Fig. 2 with image from Fernandez et al., 2018
GABAergic neuron GFP expression decreased amount, abnormal bud13ya103/ya103; nns9Tg; ot1Tg standard conditions Fig. 2 with image from Fernandez et al., 2018
Citations
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