CRISPR

CRISPR3-macf1a

ID

ZDB-CRISPR-180226-3

Name

CRISPR3-macf1a

Previous Names

Intron8 -Reverse Strand (1)

Target

macf1a (1)

Sequence

5' - TACAGATGGCCACAGGTAAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
p2CH1CH2	macf1a

Expression

Gene expression in Wild Types + CRISPR3-macf1a

No data available

Phenotype

Phenotype resulting from CRISPR3-macf1a

No data available

Phenotype of all Fish created by or utilizing CRISPR3-macf1a

Phenotype	Fish	Conditions	Figures
oocyte stage I cell cortex ab1-buc labeling absent, abnormal	macf1a^{p2CH1CH2/p2CH1CH2}	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I mitochondrial cloud increased size, abnormal	macf1a^{p2CH1CH2/p2CH1CH2}	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I nucleus mislocalised, abnormal	macf1a^{p2CH1CH2/p2CH1CH2}	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I organelle disassembly decreased occurrence, abnormal	macf1a^{p2CH1CH2/p2CH1CH2}	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I cell cortex ab1-buc labeling absent, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I mitochondrial cloud increased size, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I nucleus mislocalised, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I polarity, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 8 from Escobar-Aguirre et al., 2017
oocyte stage I organelle disassembly decreased occurrence, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 7 from Escobar-Aguirre et al., 2017
oocyte stage I establishment of cell polarity decreased occurrence, abnormal	macf1a^sa12708/+; macf1a^p2CH1CH2/+	standard conditions	Fig. 8 from Escobar-Aguirre et al., 2017

Citations